Bradshaw R M, Williams S T
Microbios. 1976;15(59):57-65.
The fibrillar sheath surrounding spores of Streptomyces griseus was separated from spores and partially purified by differential centrifugation. The reaction of isolated sheath material to various solvents and enzymes was monitored by electron microscopy. Fibrilar elements (rodlets) were apparently resistant to most solvents and enzymes used; only acetone induced any change in their appearance. Analysis of sheath material showed that its composition differed considerably from that of spore walls, having a low content of nitrogen (1.7% w/w), amino sugars (0.067% w/w) and carbohydrate (0.036% w/w). It was suggested that the sheath may be at least partially inorganic in nature.
围绕灰色链霉菌孢子的纤维鞘通过差速离心从孢子中分离出来并部分纯化。通过电子显微镜监测分离出的鞘材料对各种溶剂和酶的反应。纤维状成分(小杆)显然对大多数使用的溶剂和酶具有抗性;只有丙酮会使其外观发生任何变化。鞘材料的分析表明,其组成与孢子壁的组成有很大差异,氮含量低(1.7% w/w)、氨基糖含量低(0.067% w/w)和碳水化合物含量低(0.036% w/w)。有人提出,鞘在本质上可能至少部分是无机的。