Valu G, Békési I, Szabó G
Acta Biol Acad Sci Hung. 1975;26(3-4):151-6.
Ribosomes from vegetative cells and spores of Streptomyces griseus have been used to prepare ribosomal proteins for polyacrylamide gel electrophoresis. Differences in the gel electrophoretic profile of proteins from vegetative cells and spore ribosomes can be detected. Spore ribosomes were stable during the isolation process, but in the case of vegetative cell ribosomes it was necessary to use protease inhibitor to obtain reproducible results. Suspensions of washed ribosomes of vegetative hyphae have higher proteolytic activity than ribosomes from spores.
来自灰色链霉菌营养细胞和孢子的核糖体已被用于制备用于聚丙烯酰胺凝胶电泳的核糖体蛋白。可以检测到营养细胞和孢子核糖体蛋白在凝胶电泳图谱上的差异。孢子核糖体在分离过程中是稳定的,但对于营养细胞核糖体,有必要使用蛋白酶抑制剂以获得可重复的结果。营养菌丝洗涤后的核糖体悬浮液比孢子核糖体具有更高的蛋白水解活性。