Mazumdar S, SenGupta S K, Param R, Sinha S N
Department of Basic Medical Sciences, Faculty of Medicine, University of Papua New Guinea, Boroko.
Int J Oral Maxillofac Surg. 1993 Oct;22(5):301-5. doi: 10.1016/s0901-5027(05)80521-1.
The present investigation was undertaken to assess the binding characteristics of eight different lectins to normal oral mucosa (11 cases), leukoplakia with varying degrees of dysplasia (five cases), and oral mucosal squamous cell carcinoma (12 cases) by the use of biotinylated lectins and avidin biotin peroxidase complexes. The lectins employed were soybean (SBA), peanut (PNA), Dolichos biflorus (DBA), concanavalin A (Con A), wheat germ (WGA), Ulex europaeus (UEA), Ricinus communis (RCA), and Lotus tetragonolobus (LTA). It was observed that SBA, DBA, WGA, UEA, RCA, and LTA showed very strong to strong binding in healthy oral mucosa but no or very weak binding in squamous cell carcinoma. On the contrary, PNA showed weak binding to normal mucosal epithelial cells but showed strong binding to malignant cells. The dysplastic mucosa had an intermediate binding pattern. The lectin Con A was not bound at all or seen in very low concentration in the malignant cells and dysplastic epithelium, but it showed weak binding in the normal mucosa. Hence, we conclude that lectins may be utilized as probes to determine the dysplastic and malignant status of the oral mucosal epithelium.
本研究旨在通过使用生物素化凝集素和抗生物素蛋白-生物素过氧化物酶复合物，评估八种不同凝集素与正常口腔黏膜（11例）、不同程度发育异常的白斑（5例）和口腔黏膜鳞状细胞癌（12例）的结合特性。所使用的凝集素为大豆凝集素（SBA）、花生凝集素（PNA）、双花扁豆凝集素（DBA）、伴刀豆球蛋白A（Con A）、麦胚凝集素（WGA）、荆豆凝集素（UEA）、蓖麻凝集素（RCA）和四角豆凝集素（LTA）。观察到SBA、DBA、WGA、UEA、RCA和LTA在健康口腔黏膜中显示出非常强至强的结合，但在鳞状细胞癌中无结合或结合非常弱。相反，PNA与正常黏膜上皮细胞结合较弱，但与恶性细胞结合较强。发育异常的黏膜具有中间结合模式。凝集素Con A在恶性细胞和发育异常的上皮细胞中根本不结合或仅以非常低的浓度出现，但在正常黏膜中显示出弱结合。因此，我们得出结论，凝集素可作为探针用于确定口腔黏膜上皮的发育异常和恶性状态。
