Giaimis J, Lombard Y, Fonteneau P, Muller C D, Levy R, Makaya-Kumba M, Lazdins J, Poindron P
Université Louis Pasteur, Département d'Immunologie, d'Immunopharmacologie et de Pathologie, Illkirch, France.
J Leukoc Biol. 1993 Dec;54(6):564-71. doi: 10.1002/jlb.54.6.564.
We studied the involvement of lectin-like receptors in phagocytosis of unopsonized heat-killed yeast (Saccharomyces cerevisiae) by murine macrophage-like cell lines and murine peritoneal resident macrophages. For this purpose we used a technique that allowed us to discriminate ingested and adsorbed heat-killed yeast. The internalization can be partly inhibited by soluble polyosides such as laminarin (beta-glucan) or alpha-mannan. However, when they were used together (0.4 mg/ml alpha-mannan and 0.4 mg/ml laminarin), almost complete inhibition of phagocytosis was obtained. These observations suggest that phagocytosis of unopsonized heat-killed yeast by murine macrophage-like cell lines as well as murine peritoneal resident macrophages is mediated by both mannose and beta-glucan receptors. The respective activity of these two types of receptors is a function of in vitro cell differentiation. To achieve maximal phagocytosis of unopsonized heat-killed yeast, coexpression of both mannose and beta-glucan receptors is required.
我们研究了凝集素样受体在小鼠巨噬细胞样细胞系和小鼠腹腔常驻巨噬细胞对未调理的热灭活酵母（酿酒酵母）吞噬作用中的参与情况。为此，我们使用了一种技术，使我们能够区分摄入的和吸附的热灭活酵母。内化作用可被可溶性多聚糖如海带多糖（β-葡聚糖）或α-甘露聚糖部分抑制。然而，当它们一起使用时（0.4mg/mlα-甘露聚糖和0.4mg/ml海带多糖），几乎完全抑制了吞噬作用。这些观察结果表明，小鼠巨噬细胞样细胞系以及小鼠腹腔常驻巨噬细胞对未调理的热灭活酵母的吞噬作用是由甘露糖受体和β-葡聚糖受体共同介导的。这两种类型受体的各自活性是体外细胞分化的一个函数。为了实现对未调理的热灭活酵母的最大吞噬作用，需要甘露糖受体和β-葡聚糖受体的共表达。
