Ghoneim F A, Fateen B A, Rashad M, Abbas M O, Zaki A, Shahwan A A, Mohamed T A, el-Shabrawy L E
Department of Obst. Gynecol., Faculty of Medicine, Ain Shams University.
J Egypt Public Health Assoc. 1993;68(1-2):49-62.
This study was performed on two groups of parturient women. The first group included 20 parturient women with premature rupture of membranes (PROM) and the second group included 20 parturient women with intact membranes. From each case a specimen was taken by a sterile swab from the posterior fornix for bacteriological examination to isolate and identify both aerobic and anaerobic vaginal organisms. Also a specimen 4 x 4 cm was cut from chorioamniotic membrane at site of rupture for histological examination including routine hematoxylin and eosin staining and silver impregnation technique (Gordon & Sweet method) for identification of reticular-argyrophilic fibers (collagen III). It has been found that collagen type III was significantly decreased in amniotic membranes in cases with PROM, its decrease can be considered as a predisposing factor of PROM. The beta-hemolytic streptococci showed a significant increase in vaginal swabs taken from cases with PROM. No linking correlation was found between types of organisms in vagina and decreased collagen type III in amniotic membrane with premature rupture. Polymorphonuclear leukocytes showed no significant increase in amniotic membrane belonging to cases of PROM.
本研究对两组产妇进行。第一组包括20例胎膜早破的产妇，第二组包括20例胎膜完整的产妇。从每个病例中，用无菌拭子从后穹窿采集标本进行细菌学检查，以分离和鉴定需氧和厌氧阴道微生物。此外，在破裂部位从绒毛羊膜上切取4×4 cm的标本进行组织学检查，包括常规苏木精和伊红染色以及用于鉴定网状嗜银纤维（Ⅲ型胶原）的银浸染技术（戈登和斯威特法）。研究发现，胎膜早破病例的羊膜中Ⅲ型胶原显著减少，其减少可被视为胎膜早破的一个易感因素。β溶血性链球菌在胎膜早破病例的阴道拭子中显著增加。在胎膜早破的情况下，未发现阴道微生物类型与羊膜中Ⅲ型胶原减少之间存在关联。胎膜早破病例的羊膜中多形核白细胞无显著增加。
