Shirai M, Fujise H, Masaoka T, Takeda S, Tsuboi T, Akahori F
Department of Veterinary Pharmacology, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
J Toxicol Sci. 1993 Aug;18(3):167-70. doi: 10.2131/jts.18.3_167.
Pulmonary fibrosis induction by paraquat was studied histometrically using an image analyzer. Rats were given a subcutaneous injection of paraquat (7 or 25 mg/kg) and autopsied 3, 6 or 9 days later. The percentage of the area of connective fibers per unit area, and lung prolyl hydroxylase activity, as a biochemical parameter, were measured. Rats treated with 25 mg/kg of paraquat showed an increase in the percentage of the area of the connective fibers per unit area and also an increase in lung prolyl hydroxylase activity 6 days later. Thus, pulmonary fibrosis was confirmed both morphologically and biochemically. It is concluded that histometric examination using an image analyzer provides a very accurate method for the evaluation of pulmonary fibrosis.
使用图像分析仪通过组织计量学方法研究了百草枯诱导的肺纤维化。给大鼠皮下注射百草枯（7或25mg/kg），并在3、6或9天后进行尸检。测量了每单位面积结缔组织纤维面积的百分比以及作为生化参数的肺脯氨酰羟化酶活性。用25mg/kg百草枯处理的大鼠在6天后每单位面积结缔组织纤维面积的百分比增加，肺脯氨酰羟化酶活性也增加。因此，在形态学和生物化学方面均证实了肺纤维化。得出的结论是，使用图像分析仪进行组织计量学检查为评估肺纤维化提供了一种非常准确的方法。
