Cameron H A, Woolley C S, Gould E
Laboratory of Neuroendocrinology, Rockefeller University, NY 10021.
Brain Res. 1993 May 21;611(2):342-6. doi: 10.1016/0006-8993(93)90524-q.
Several lines of evidence indicate that cell birth in the adult rat dentate gyrus is regulated by adrenal steroids. The expression of adrenal steroid receptors by mitotic cells in the dentate gyrus would support the hypothesis that these hormones act directly on granule cell progenitors. We performed a survival time course of in vivo [3H]thymidine autoradiography combined with immunohistochemistry for mineralocorticoid receptors (MR) and glucocorticoid receptors (GR) and found that very few [3H]thymidine labeled mitotic cells express these receptors. By 4 weeks following [3H]thymidine administration, the vast majority of [3H]thymidine labeled cells were immunoreactive for MR and GR. These results suggest that adrenal steroids do not act directly on granule cell progenitors in the adult rat dentate gyrus.
多条证据表明，成年大鼠齿状回中的细胞生成受肾上腺类固醇调节。齿状回中有丝分裂细胞对肾上腺类固醇受体的表达支持了这样一种假说，即这些激素直接作用于颗粒细胞祖细胞。我们进行了体内[3H]胸腺嘧啶核苷放射自显影的存活时间进程研究，并结合盐皮质激素受体（MR）和糖皮质激素受体（GR）的免疫组织化学分析，发现极少有[3H]胸腺嘧啶核苷标记的有丝分裂细胞表达这些受体。在给予[3H]胸腺嘧啶核苷4周后，绝大多数[3H]胸腺嘧啶核苷标记的细胞对MR和GR呈免疫反应性。这些结果表明，肾上腺类固醇并不直接作用于成年大鼠齿状回中的颗粒细胞祖细胞。
