Detection of Epstein-Barr virus by polymerase chain reaction in transbronchial biopsies of lung transplant recipients: evidence of infection?

Thirty-nine paraffin-embedded transbronchial biopsies from ten lung transplant recipients were evaluated by the polymerase chain reaction for the presence of Epstein-Barr virus (EBV). Six of these patients had positive EBV serology. Nine biopsies from nine non-transplant patients with nonspecific interstitial pneumonitis were studied as a control group. Eight biopsies from four of the ten transplant patients (40%) were positive for EBV by polymerase chain reaction, utilizing 40 cycles and two sets of primers (501/502) complementary to sequences within the Bam H1 W region of the viral genome. Five of these eight positive biopsies had been diagnosed as mild acute cellular rejection based on clinicopathologic criteria. Three of the four EBV-positive patients showed clinical improvement with antiviral agents prescribed for concomitant cytomegalovirus infection. Our data demonstrate the potential of polymerase chain reaction to detect small quantities of EBV in transbronchial biopsies from lung transplant recipients. Such findings should be interpreted cautiously in the evaluation of lung transplant recipients, since EBV can be detected in the absence of a lymphoproliferative disorder or an active pneumonitis caused by this virus.