Sommerfelt H, Bhan M K, Srivastava R, Bhatnagar S
Centre for International Health, University of Bergen, Haukeland Hospital, Norway.
Scand J Infect Dis. 1993;25(4):457-63. doi: 10.3109/00365549309008527.
The simplicity of enterotoxigenic Escherichia coli (ETEC) stool blot hybridization, where the total bacterial growth of a fecal inoculum is examined directly for the presence of enterotoxin genes, has been marred by reports of unsatisfactory sensitivity and/or specificity. To assess the accuracy of stool blot hybridization and to study the effect of varying proportions of ETEC among fecal E. coli (ETEC/E. coli) on test performance, a detailed 'blind' study of 166 stool specimens from children with diarrhea was performed. Oligonucleotide probes were found to be superior to polynucleotide probes, having a sensitivity of 80%, a specificity of 99%, a positive predictive value of 89% and a negative predictive value of 97%. The sensitivity was found to be 100% when ETEC/E. coli > 2/12, as compared with 20% when ETEC/E. coli < or = 2/12 (p = 0.001), showing that the proportion of ETEC among fecal E. coli is of paramount importance for test sensitivity.
产肠毒素大肠杆菌（ETEC）粪便印迹杂交法操作简单，可直接检测粪便接种物中的细菌总数以确定是否存在肠毒素基因，但已有报道称其敏感性和/或特异性不尽人意。为评估粪便印迹杂交法的准确性，并研究粪便大肠杆菌中不同比例的ETEC（ETEC/大肠杆菌）对检测性能的影响，对166例腹泻儿童的粪便标本进行了详细的“盲法”研究。发现寡核苷酸探针优于多核苷酸探针，其敏感性为80%，特异性为99%，阳性预测值为89%，阴性预测值为97%。当ETEC/大肠杆菌>2/12时，敏感性为100%；而当ETEC/大肠杆菌≤2/12时，敏感性为20%（p = 0.001），这表明粪便大肠杆菌中ETEC的比例对检测敏感性至关重要。
