寡核苷酸基因探针可直接在粪便涂片检测产肠毒素大肠杆菌。
Oligonucleotide gene probes may detect enterotoxigenic Escherichia coli directly in fecal smears.
作者信息
Sommerfelt H, Bhan M K, Brahmadattan K N, Srivastava R, Suresh M, Bjorvatn B
机构信息
Medical Department B, University of Bergen, Norway.
出版信息
APMIS. 1989 Jan;97(1):91-4.
Diarrhoeal stools from 86 Indian children were examined for the presence of enterotoxigenic Escherichia coli (ETEC) by DNA hybridization on bacterial colonies as well as directly on fecal smears. Colony hybridization showed that 8 of the samples contained ETEC. Only in three of these specimens were all the colonies tested positive for ETEC. These samples were correctly identified by the stool smear hybridization assay. The remaining 5 specimens produced enterotoxigenic as well as nonenterotoxigenic colonies. These samples were all negative by the stool smear hybridization technique. Methodological improvements of the stool smear assay may provide a substantially simplified method for the otherwise cumbersome identification of ETEC.
通过对细菌菌落以及直接对粪便涂片进行DNA杂交，检测了86名印度儿童腹泻粪便中是否存在产肠毒素大肠杆菌（ETEC）。菌落杂交显示，8份样本含有ETEC。在这些样本中，只有3份样本的所有菌落检测出ETEC呈阳性。这些样本通过粪便涂片杂交试验被正确鉴定。其余5份样本产生了产肠毒素和非产肠毒素的菌落。这些样本通过粪便涂片杂交技术检测均为阴性。粪便涂片检测方法的改进可能会为原本繁琐的ETEC鉴定提供一种大幅简化的方法。
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