Evans L A, McHugh T M, Stites D P, Levy J A
J Immunol. 1987 May 15;138(10):3415-8.
Isolates of HIV showed distinct differences in the ability to replicate in continuous human hematopoietic cell lines. Moreover, although all PMC cultures obtained from healthy individuals could be infected with HIV, considerable variation in the amount of virus released from different PMC cultures was observed. These biological properties of HIV could not be correlated with clinical state, binding properties of the virus isolates to target cells, or differences in target cell CD4 antigen expression. Some isolates of HIV that could not directly infect the HUT-78 cell line showed productive infection when PMC infected with these viruses were added to this human T cell line. These observations emphasize the importance of cell to cell contact in the spread of virus. The results demonstrate for the first time the differences in the host range specificity of HIV isolates in several individual PMC cultures, and indicate that the optimal isolation of HIV is achieved with normal human PMC rather than established human cell lines.
HIV分离株在连续人类造血细胞系中的复制能力存在明显差异。此外，尽管从健康个体获得的所有外周血单核细胞（PMC）培养物都能被HIV感染，但不同PMC培养物释放的病毒量存在相当大的差异。HIV的这些生物学特性与临床状态、病毒分离株与靶细胞的结合特性或靶细胞CD4抗原表达的差异无关。一些不能直接感染HUT - 78细胞系的HIV分离株，当将感染这些病毒的PMC添加到这个人T细胞系中时，会出现有效感染。这些观察结果强调了细胞间接触在病毒传播中的重要性。结果首次证明了HIV分离株在几种个体PMC培养物中的宿主范围特异性存在差异，并表明用正常人PMC而非已建立的人类细胞系能实现HIV的最佳分离。
