Tugizov S M, Savchenkova I P, Grabovskaya I L, Makarova N E, Eraizer T L, Revazova E P, Kushch A A
D.I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, Moscow.
Virus Res. 1993 Nov;30(2):189-203. doi: 10.1016/0168-1702(93)90006-9.
Mutant clones of human hepatocarcinoma PLC-PRF-5 cells carrying a hepatitis B virus (HBV) genome have been obtained using selection for resistance to the toxic action of a variety of preparations to induce cell differentiation. The clones differed in various features such as expression of alpha-fetoprotein (AFP) and albumin as well as in growth rates, ability to grow in semisolid media and to be cloned in agar. Expression of the surface antigen (HBsAg) was significantly increased in mutant cells exhibiting differentiation features in contrast to the parental cells. In addition, the core antigen (HBcAg), which was silent in the original cells, was detected in some clones. There was no temporal correlation between the peak of enhanced expression of HBsAg and activation of HBcAg observed at different life periods of each clone. Evidence of cell fusion in cell culture such as premature chromatin condensation and increased numbers of binucleate cells was detected in clones with differentiation features and an increased level of viral gene expression. The approach used in this study can be used to develop cell lines of the same origin with different degrees of differentiation whilst maintaining HBV expression. This model system may be useful in the study of HBV.
通过选择对多种诱导细胞分化制剂的毒性作用具有抗性，已获得携带乙型肝炎病毒（HBV）基因组的人肝癌PLC-PRF-5细胞的突变克隆。这些克隆在各种特征上存在差异，例如甲胎蛋白（AFP）和白蛋白的表达，以及生长速率、在半固体培养基中生长和在琼脂中克隆的能力。与亲代细胞相比，在表现出分化特征的突变细胞中，表面抗原（HBsAg）的表达显著增加。此外，在一些克隆中检测到在原始细胞中沉默的核心抗原（HBcAg）。在每个克隆的不同生命周期观察到的HBsAg表达增强峰值与HBcAg激活之间没有时间相关性。在具有分化特征和病毒基因表达水平升高的克隆中，检测到细胞培养中细胞融合的证据，如染色质过早凝聚和双核细胞数量增加。本研究中使用的方法可用于开发具有不同分化程度的同源细胞系，同时保持HBV表达。该模型系统可能对HBV的研究有用。
