Matus Z, Molnár P, Szabó L G
Pécsi Orvostudományi Egyetem Orvosi Kémiai Intézete, Pécs.
Acta Pharm Hung. 1993 Sep;63(5):247-56.
Various use of the oil-pumpkin offers reason for the phytochemical analysis of seed-meal's carotene pigments. Column chromatography was performed on the adsorbents MgO, Celite and CaCO3 with hexane and benzene as eluents. HPLC separation of different pigments was carried out on a 6 microns reverse phase packing with a ternary gradient elution method using a diode-array detector. The main components of the press-residue were lutein [3,3'-dihydroxy-alpha-carotene = (3R,3'R,6'R)-beta,epsilon-carotene-3,3'-diol; 52.5%] and beta-carotene (beta,epsilon-carotene; 10.1%). In addition to the above-mentioned pigments it was successful to reveal the presence of violaxanthin, luteoxanthin, auroxanthin epimers, lutein epoxide, flavoxanthin, chrysanthemaxanthin, 9(9')-cis-lutein, 13(13')-cis-lutein, 15-cis-lutein (central-cis)-lutein, alpha-cryptoxanthin, beta-cryptoxanthin and alpha-carotene (beta,epsilon-carotene) in small quantities.
油南瓜的多种用途为对籽粕中胡萝卜素色素进行植物化学分析提供了理由。以氧化镁、硅藻土和碳酸钙为吸附剂，以己烷和苯为洗脱剂进行柱色谱分析。采用三元梯度洗脱法，使用二极管阵列检测器，在6微米反相填料上对不同色素进行高效液相色谱分离。压榨残渣的主要成分是叶黄素[3,3'-二羟基-α-胡萝卜素 = (3R,3'R,6'R)-β,ε-胡萝卜素-3,3'-二醇；52.5%]和β-胡萝卜素(β,ε-胡萝卜素；10.1%)。除上述色素外，还成功检测出少量的紫黄质、黄体黄质、金盏花黄质差向异构体、环氧叶黄素、黄素黄质、菊花黄质、9(9')-顺式叶黄素、13(13')-顺式叶黄素、15-顺式叶黄素(中心-顺式)-叶黄素、α-隐黄质、β-隐黄质和α-胡萝卜素(β,ε-胡萝卜素)。
