Plommet M, Plommet A M
Ann Rech Vet. 1976;7(1):1-8.
Eighteen 25 month old cows were vaccinated once either with 10(6) to 10(10) living bacteria of Brucella abortus B 19 by the conjunctival route, or with 2.5 to 5 X 10(10) formalin-killed bacteria of the same strain by subcutaneous injection. Fifteen days post-vaccination, low but definite agglutination titers were present in sera of cattle receiving 10(10) living bacteria by the conjunctival route, whereas high titers were observed in the control group given subcutaneous injection of killed bacteria (table I). Agglutination reactions were negative in sera from all cattle receiving less than 10(10) bacteria, and complement fixation tests were negative with all sera. Seventy-one 4 month old claves were vaccinated by the conjunctival route with different doses (10(6) to 10(10)) of living bacteria, either freshly prepared or lyophilized. At autopsy about 15 or about 30 days later, the parotid, submaxillary and retropharyngeal lymph nodes were removed and cultured on Brucella selective medium. Blood samples were taken at autopsy for testing by agglutination, complement fixation, Coombs test and (for some samples) by the Rose Bengal plate test. Colonization of at least one lymph node was observed in 26 of 34 calves given 10(9) bacteria, in all 9 calves given 5 X 10(9) bacteria and in all 12 calves given 10(10) bacteria. Serological responses were demonstrated rarely, and then only in agglutination and Coombs tests. The 5 X 10(9) dose, wich following conjunctival inoculation consistently colonized the regional lymph nodes without inducing significant serological response, would be the most suitable dose for the vaccination of calves and cows.
18头25月龄的母牛，通过结膜途径用10⁶至10¹⁰个流产布鲁氏菌B19活菌进行一次接种，或通过皮下注射用2.5至5×10¹⁰个同一菌株的福尔马林灭活菌进行一次接种。接种后15天，通过结膜途径接种10¹⁰个活菌的牛血清中出现了低但确定的凝集效价，而皮下注射灭活菌的对照组中观察到高效价（表I）。所有接受少于10¹⁰个细菌的牛血清中的凝集反应均为阴性，所有血清的补体结合试验也为阴性。71头4月龄的犊牛通过结膜途径用不同剂量（10⁶至10¹⁰）的新鲜制备或冻干的活菌进行接种。在约15天或约30天后进行尸检时，取出腮腺、颌下和咽后淋巴结并在布鲁氏菌选择性培养基上培养。尸检时采集血样进行凝集试验、补体结合试验、库姆斯试验以及（部分样本）玫瑰红平板试验检测。在接种10⁹个细菌的34头犊牛中有26头、接种5×10⁹个细菌的所有9头犊牛以及接种10¹⁰个细菌的所有12头犊牛中观察到至少一个淋巴结有细菌定植。血清学反应很少出现，且仅在凝集试验和库姆斯试验中出现。结膜接种后5×10⁹这个剂量能持续在局部淋巴结定植且不引起明显的血清学反应，将是犊牛和母牛接种的最合适剂量。
