Dahlgren C, Follin P, Lundqvist H, Sjölin C
Department of Medical Microbiology and Immunology, University of Göteborg, Sweden.
Anal Biochem. 1993 Oct;214(1):284-8. doi: 10.1006/abio.1993.1489.
Using slot blot, we show that myeloperoxidase (MPO), a constituent of azurophil granules of neutrophil polymorphonuclear leukocytes, can be measured quantitatively using a commercially available chemiluminescence kit, originally developed for detection of specific proteins on Western blots. MPO is determined through its ability to catalyze the oxidation of luminol, resulting in the emission of light which is recorded on a photographic film. The sensitivity of the method is high and allows MPO from less than 100 cells to be detected. This method was used to determine MPO in exudate fluid and in neutrophil fractions following disintegration and subcellular fractionation of the postnuclear supernatant on two-layer Percoll gradients.
通过狭缝印迹法，我们发现髓过氧化物酶（MPO），即中性多形核白细胞嗜天青颗粒的一种成分，可使用一种市售的化学发光试剂盒进行定量检测，该试剂盒最初是为在蛋白质印迹法中检测特定蛋白质而开发的。MPO通过其催化鲁米诺氧化的能力来测定，氧化过程会产生光发射，并记录在摄影胶片上。该方法灵敏度高，能够检测到少于100个细胞中的MPO。此方法用于测定渗出液以及在两层Percoll梯度上对核后上清液进行裂解和亚细胞分级分离后获得的中性粒细胞组分中的MPO。
