Christensen L R, Møllgård K, Kjaer I, Janas M S
Department of Orthodontics, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Denmark.
Anat Embryol (Berl). 1993 Sep;188(3):247-55. doi: 10.1007/BF00188216.
Evidence is accumulating that nerve growth factor receptor (NGF-R or p75NGFR) can mediate cell growth and differentiation of non-neuronal cells. NGF-R expression was studied in developing teeth of human embryos and fetuses between the 6th and 18th weeks of gestation, using a monoclonal mouse-anti-human NGF-R antibody. In contrast to earlier findings in rodents, the NGF-R expression of the human dental papilla was found to be transient. NGF-R was present in the condensing ecto-mesenchymal cells of the dental papilla in the early cap stage tooth germ. In later developmental stages, a shift of the NGF-R expression from the papilla to the cytoplasmic membrane of the inner enamel epithelium (IEE) was demonstrated. As in rodent odontogenesis, the NGF-R immunoreactivity of the IEE remained until the odontoblasts started secretion of predentinal matrix in the late bell state. The mitotic activity in the IEE was detected by an antibody against proliferating cell nuclear antigen (PCNA) and showed that the NGF-R expression of the IEE decreased as the cell proliferation ceased. We propose that NGF-R may, be involved in differential and/or proliferative events of human odontogenesis.
越来越多的证据表明，神经生长因子受体（NGF-R或p75NGFR）可以介导非神经元细胞的生长和分化。使用单克隆小鼠抗人NGF-R抗体，研究了妊娠第6至18周的人类胚胎和胎儿发育牙齿中的NGF-R表达。与早期在啮齿动物中的发现相反，发现人类牙乳头中的NGF-R表达是短暂的。在帽状期早期牙胚的牙乳头凝聚外间充质细胞中存在NGF-R。在后期发育阶段，证明了NGF-R表达从牙乳头转移到内釉上皮（IEE）的细胞膜。与啮齿动物牙发生一样，IEE的NGF-R免疫反应性一直保持到成牙本质细胞在钟状期末期开始分泌前期牙本质基质。通过抗增殖细胞核抗原（PCNA）抗体检测IEE中的有丝分裂活性，结果显示随着细胞增殖停止，IEE的NGF-R表达降低。我们认为NGF-R可能参与人类牙发生的分化和/或增殖事件。
