葡萄糖、生长温度和pH值对单核细胞增生李斯特菌中李斯特菌溶血素O产生的影响。
Effects of glucose, growth temperature, and pH on listeriolysin O production in Listeria monocytogenes.
作者信息
Datta A R, Kothary M H
机构信息
Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.
出版信息
Appl Environ Microbiol. 1993 Oct;59(10):3495-7. doi: 10.1128/aem.59.10.3495-3497.1993.
Expression of listeriolysin O of Listeria monocytogenes as a function of different growth conditions was studied by performing a direct hemolysin assay, immunoblotting experiments, and an enzyme-linked immunosorbent assay. Expression of listeriolysin O was reduced at a lower growth temperatures (26 degrees C) and at higher glucose concentrations (> or = 0.3%) in the growth media. The effect of glucose appeared to be due to a change in the pH of the growth media.
通过进行直接溶血素测定、免疫印迹实验和酶联免疫吸附测定，研究了不同生长条件下单增李斯特菌溶血素O的表达情况。溶血素O的表达在较低生长温度（26℃）和生长培养基中较高葡萄糖浓度（≥0.3%）时降低。葡萄糖的影响似乎是由于生长培养基pH值的变化。
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