冷藏条件下棘阿米巴中微粒体δ12（ω6）-去饱和酶活性的快速诱导
Rapid induction of microsomal delta 12(omega 6)-desaturase activity in chilled Acanthamoeba castellanii.
作者信息
Jones A L, Lloyd D, Harwood J L
机构信息
Department of Biochemistry, School of Pure and Applied Biology, University of Wales College of Cardiff, U.K.
出版信息
Biochem J. 1993 Nov 15;296 ( Pt 1)(Pt 1):183-8. doi: 10.1042/bj2960183.
Abstract
The activity of microsomal delta 12-desaturase in Acanthamoeba castellanii was increased after growing cultures were chilled from the optimal growth temperature (30 degrees C) to 15 degrees C. This increase was detectable in microsomes isolated from organisms subjected to only 10 min chilling. The mechanism of induction was investigated. The increase in activity on chilling was greatly reduced when protein synthesis was blocked before the temperature shift. Thus the major mechanism for the induction of delta 12-desaturase is increased protein synthesis. delta 12-Desaturase activity was higher when assayed at 20 degrees C than when assayed at 30 degrees C, but these changes were not due to the increased solubility of O2 at 20 degrees C. The major substrate of delta 12-desaturase was found to be 1-acyl-2-oleoyl phosphatidylcholine.
摘要
将生长中的卡氏棘阿米巴培养物从最佳生长温度（30摄氏度）冷却至15摄氏度后，微粒体Δ12-去饱和酶的活性增加。从仅经过10分钟冷却处理的生物体中分离出的微粒体中即可检测到这种增加。对诱导机制进行了研究。当在温度转变前阻断蛋白质合成时，冷却时活性的增加会大大降低。因此，诱导Δ12-去饱和酶的主要机制是蛋白质合成增加。在20摄氏度下测定时，Δ12-去饱和酶的活性高于在30摄氏度下测定时，但这些变化并非由于20摄氏度下氧气溶解度增加所致。发现Δ12-去饱和酶的主要底物是1-酰基-2-油酰磷脂酰胆碱。
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本文引用的文献
1
A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
2
Temperature-induced membrane-lipid adaptation in Acanthamoeba castellanii.棘阿米巴中温度诱导的膜脂适应性
Biochem J. 1993 Feb 15;290 ( Pt 1)(Pt 1):273-8. doi: 10.1042/bj2900273.
3
4
The occurrence of direct desaturation of phospholipid acyl chain in Tetrahymena pyriformis. Thermal adaptation of membrane phospholipid.梨形四膜虫中磷脂酰基链的直接去饱和现象。膜磷脂的热适应性。
Biochim Biophys Acta. 1980 May 28;618(2):214-22.
5
Functional association of a monoacylglycerophosphocholine acyltransferase and the oleoylglycerophosphocholine desaturase in microsomes from developing leaves.发育叶片微粒体中甘油单酯磷酸胆碱酰基转移酶与油酰甘油磷酸胆碱去饱和酶的功能关联
Eur J Biochem. 1984 Mar 1;139(2):373-9. doi: 10.1111/j.1432-1033.1984.tb08016.x.
6
The effect of hypolipidemic drugs WY14643 and DH990, and lysophospholipids on the metabolism of oleate in plants.降血脂药物WY14643和DH990以及溶血磷脂对植物中油酸代谢的影响。
Arch Biochem Biophys. 1984 Jan;228(1):185-96. doi: 10.1016/0003-9861(84)90060-2.
7
Effects of temperature acclimation on Neurospora phospholipids. Fatty acid desaturation appears to be a key element in modifying phospholipid fluid properties.温度驯化对粗糙脉孢菌磷脂的影响。脂肪酸去饱和作用似乎是改变磷脂流体性质的关键因素。
Biochim Biophys Acta. 1981 Sep 24;665(3):399-407. doi: 10.1016/0005-2760(81)90252-6.
8
Lipid involvement in oleoyl CoA desaturase activity of Fusarium oxysporum microsomes.脂质参与尖孢镰刀菌微粒体中油酰辅酶A去饱和酶的活性。
Can J Biochem. 1980 Feb;58(2):97-102. doi: 10.1139/o80-014.
9
Effect of low temperature on fatty acid biosynthesis in seeds.低温对种子中脂肪酸生物合成的影响。
Biochim Biophys Acta. 1969 Jul 29;187(1):13-8. doi: 10.1016/0005-2760(69)90127-1.
10
The biosynthesis of unsaturated fatty acids by bacilli. IV. Temperature-mediated control mechanisms.芽孢杆菌属细菌对不饱和脂肪酸的生物合成。IV. 温度介导的控制机制。
J Biol Chem. 1972 Jun 10;247(11):3511-9. doi: 10.2172/4738479.
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