Wasowicz W, Nève J, Peretz A
Laboratory of Pharmaceutical Chemistry, Free University of Brussels, Belgium.
Clin Chem. 1993 Dec;39(12):2522-6.
A simple, reliable, and reproducible fluorometric method for measuring thiobarbituric acid-reactive substances (TBARS) in serum is proposed, based on the reaction between malondialdehyde (MDA) and thiobarbituric acid. Formation of TBARS was complete at pH 2.4-2.6, but extraction with n-butanol proved complete only at lower pH, i.e., 1.6-1.7. Analytical recoveries of MDA added to serum were 94%-101%; within- and between-run CVs were 2.4-3.6% and 4.6-5.5%; and the detection limit for TBARS in serum was 0.10 mumol/L. Optimized conditions included: (a) collection of either serum or heparinized plasma, (b) preservation from in vitro autoxidation by glutathione and EDTA, and (c) storage at -20 degrees C up to 35 days. The mean (+/- SD) TBARS concentration in 47 healthy adults was 1.01 (0.21) mumol/L; no sex-related difference was observed. Higher concentrations were measured in patients with renal insufficiency undergoing hemodialysis and in patients with insulin-dependent diabetes, chronic pancreatitis, or liver cirrhosis.
基于丙二醛（MDA）与硫代巴比妥酸之间的反应，提出了一种简单、可靠且可重复的荧光法，用于测定血清中的硫代巴比妥酸反应性物质（TBARS）。TBARS的形成在pH 2.4 - 2.6时完成，但正丁醇萃取仅在较低pH值（即1.6 - 1.7）时才完全。添加到血清中的MDA的分析回收率为94% - 101%；批内和批间变异系数分别为2.4% - 3.6%和4.6% - 5.5%；血清中TBARS的检测限为0.10μmol/L。优化条件包括：（a）收集血清或肝素化血浆，（b）通过谷胱甘肽和乙二胺四乙酸防止体外自氧化，以及（c）在-20℃下储存长达35天。47名健康成年人的平均（±标准差）TBARS浓度为1.01（0.21）μmol/L；未观察到性别差异。在接受血液透析的肾功能不全患者以及胰岛素依赖型糖尿病、慢性胰腺炎或肝硬化患者中测得的浓度较高。
