Rombach E, Stetler D A, Brown J C
Department of Microbiology, University of Kansas, Lawrence 66045.
Clin Exp Immunol. 1993 Dec;94(3):466-72.
Systemic lupus erythematosus (SLE)-like anti-IgG Fab autoantibodies (autoAb) were induced in rabbits by immunization with either human, mouse or rabbit anti-DNA Ab. In direct-binding radioimmunoassay (RIA), affinity-purified anti-normal rabbit (NR) Fab autoAb cross-reacted with normal mouse (NM) Fab, ssDNA (but not dsDNA), poly(dA,dT), and RNA polymerase I (RPI). Affinity-purified anti-NM IgG Ab isolated from the same antisera cross-reacted with NR Fab, ssDNA and RPI. In inhibition RIA, soluble NR Fab inhibited anti-NR Fab binding to NR Fab and ssDNA, but enhanced binding to RPI. In contrast, ssDNA or RPI inhibitors had no effect upon autoAb binding to NR Fab. Anti-DNA, anti-RPI and anti-RPI 190 kD subunit autoAb, induced by immunization with lupus mouse anti-DNA Ab, also reacted with NM Fab, but were idiotypically specific for lupus mouse anti-DNA Fab. Further, rabbit anti-DNA and anti-RPI IgG autoAb, induced by immunization with rabbit anti-DNA IgG, were each idiotypically specific for homologous and autologous rabbit anti-ssDNA Fab. Together, these data provide evidence that anti-DNA, anti-RPI and anti-Fab autoAb are linked in a complex, multiple-specific and perhaps regulatory, immune response idiotype network in SLE.
通过用人、小鼠或兔抗DNA抗体免疫兔子，可诱导出系统性红斑狼疮（SLE）样抗IgG Fab自身抗体（自身抗体）。在直接结合放射免疫分析（RIA）中，亲和纯化的抗正常兔（NR）Fab自身抗体与正常小鼠（NM）Fab、单链DNA（而非双链DNA）、聚（dA，dT）和RNA聚合酶I（RPI）发生交叉反应。从相同抗血清中分离出的亲和纯化抗NM IgG抗体与NR Fab、单链DNA和RPI发生交叉反应。在抑制性RIA中，可溶性NR Fab抑制抗NR Fab与NR Fab和单链DNA的结合，但增强其与RPI的结合。相反，单链DNA或RPI抑制剂对自身抗体与NR Fab的结合没有影响。用狼疮小鼠抗DNA抗体免疫诱导产生的抗DNA、抗RPI和抗RPI 190 kD亚基自身抗体也与NM Fab反应，但对狼疮小鼠抗DNA Fab具有独特型特异性。此外，用兔抗DNA IgG免疫诱导产生的兔抗DNA和抗RPI IgG自身抗体分别对同源和自身兔抗单链DNA Fab具有独特型特异性。总之，这些数据提供了证据，表明抗DNA、抗RPI和抗Fab自身抗体在SLE的一个复杂、多特异性且可能具有调节作用的免疫反应独特型网络中相互关联。
