Abdou N I, Wall H, Lindsley H B, Halsey J F, Suzuki T
J Clin Invest. 1981 May;67(5):1297-304. doi: 10.1172/jci110158.
Regulation of serum anti-DNA antibody in systemic lupus erythematosus (SLE) by an antiidiotypic antibody was evaluated. Various sera from SLE patients in active and inactive states of their disease, as well as sera from normal individuals, were first completely depleted of anti-DNA and of DNA by affinity chromatography. The suppressive capacity of equimolar concentrations of the various depleted sera (blocking sera) on target lupus sera were determined. The target sera were from lupus patients with known DNA-binding capacity. Blocking sera from inactive SLE suppressed the binding of autologous anti-DNA antibody to [(3)H]DNA (n = 19,P < 0.01). Blocking sera from active SLE (n = 19), as well as human serum albumin, did not suppress. Sera from normal donors who had no contact with lupus patients or with lupus sera did not suppress (n = 14, P > 0.5), whereas those from normal donors who had contact with lupus patients or sera did suppress the binding (n = 5,P < 0.02). The anti-anti-DNA antibody suppressive activity in the inactive lupus serum was shown to be localized within the F(ab')(2) portion of immunoglobulin (Ig)G and could not be removed upon adsorption by normal human gammaglobulin. Furthermore, immune complexes could be detected by a Clq binding assay when the inactive lupus blocking sera were incubated with the anti-DNA antibody containing target sera. The specificity of the suppressive serum factor was shown by its inability to block the binding of tetanus toxoid to antitetanus antibody and its ability to block the binding of DNA to F(ab')(2) fragments of active lupus IgG. Regulation of serum anti-DNA antibody levels by anti-antibodies could induce and maintain disease remission in lupus patients and prevent disease expression in normals.
评估了抗独特型抗体对系统性红斑狼疮（SLE）患者血清抗DNA抗体的调节作用。首先通过亲和层析法，将处于疾病活动期和非活动期的SLE患者的各种血清以及正常个体的血清中的抗DNA和DNA完全去除。测定了等摩尔浓度的各种去除血清（封闭血清）对目标狼疮血清的抑制能力。目标血清来自已知具有DNA结合能力的狼疮患者。非活动期SLE的封闭血清抑制了自身抗DNA抗体与[³H]DNA的结合（n = 19，P < 0.01）。活动期SLE的封闭血清（n = 19）以及人血清白蛋白均无抑制作用。未接触过狼疮患者或狼疮血清的正常供体的血清无抑制作用（n = 14，P > 0.5），而接触过狼疮患者或血清的正常供体的血清则能抑制结合（n = 5，P < 0.02）。结果表明，非活动期狼疮血清中的抗抗DNA抗体抑制活性定位于免疫球蛋白（Ig）G的F(ab')₂部分，正常人γ球蛋白吸附后不能去除该活性。此外，当非活动期狼疮封闭血清与含抗DNA抗体的目标血清孵育时，通过Clq结合试验可检测到免疫复合物。抑制性血清因子的特异性表现为它不能阻断破伤风类毒素与抗破伤风抗体的结合，却能阻断DNA与活动期狼疮IgG的F(ab')₂片段的结合。抗抗体对血清抗DNA抗体水平的调节可诱导并维持狼疮患者的疾病缓解，预防正常人发病。
