Beaman L, Wisseman C L
Infect Immun. 1976 Oct;14(4):1065-70. doi: 10.1128/iai.14.4.1065-1070.1976.
Antibodies in both mouse and human Rickettsia mooseri (Rickettsia typhi) convalescent serum that were cytophilic for mouse macrophages were demonstrated by the rosette technique. Mouse peritoneal macrophages, passively sensitized with early and late serum from mice with a sublethal infection of R. mooseri, were washed and exposed to rickettsiae. Rosettes of rickettsiae were found around macrophages, maintained at 4 degrees C, which had been sensitized with immune serum (direct sensitization of macrophages), but no rosettes were found around macrophages sensitized with serum from normal mice. When the macrophages were maintained at 34 degrees C after addition of the rickettsiae, phagocytosis of rickettsiae occurred, indicating one probable role for cytophilic antibodies in typhus infections. If the rickettsiae were mixed with serum from infected mice, washed, and then added to macrophages (indirect sensitization of macrophages), more rosettes were found around the macrophages than around directly sensitized macrophages. The presence of mouse immunoglobulin G on the macrophage surface was also shown by staining living sensitized macrophages with rabbit fluroescein-conjugated anti-mouse immunoglobulin G.
采用玫瑰花结技术证实，在鼠型斑疹伤寒立克次体（斑疹伤寒立克次体）感染恢复期的小鼠和人血清中，存在对小鼠巨噬细胞具有亲细胞性的抗体。用鼠型斑疹伤寒立克次体亚致死感染小鼠的早期和晚期血清被动致敏的小鼠腹腔巨噬细胞，经洗涤后与立克次体接触。在4℃下，用免疫血清致敏的巨噬细胞周围发现了立克次体玫瑰花结（巨噬细胞的直接致敏），但在用正常小鼠血清致敏的巨噬细胞周围未发现玫瑰花结。当在加入立克次体后将巨噬细胞维持在34℃时，立克次体被吞噬，这表明亲细胞性抗体在斑疹伤寒感染中可能发挥的一种作用。如果将立克次体与感染小鼠的血清混合、洗涤，然后加入到巨噬细胞中（巨噬细胞的间接致敏），则在巨噬细胞周围发现的玫瑰花结比直接致敏的巨噬细胞周围更多。用兔荧光素偶联的抗小鼠免疫球蛋白G对活的致敏巨噬细胞进行染色，也显示了巨噬细胞表面存在小鼠免疫球蛋白G。
