鼠单克隆抗体Fab片段晶体的表征

Characterization of crystals of an Fab fragment of a murine monoclonal antibody.

作者信息

Ban N, Escobar C, Day J, Greenwood A, Larson S, McPherson A

机构信息

Department of Biochemistry, University of California, Riverside 92521.

出版信息

J Mol Biol. 1991 Dec 5;222(3):445-6. doi: 10.1016/0022-2836(91)90484-n.

DOI:10.1016/0022-2836(91)90484-n

PMID:1660922

Abstract

The Fab fragment of an antibody, made against an E2-specific feline infectious peritonitis virus neutralizing antibody, has been crystallized in a form suitable for X-ray diffraction analysis from PEG 4000 using vapor diffusion methods. The Fab fragment crystals diffract to about 2.9 A resolution and are of triclinic space group P1. Unit cell dimensions, by which the reciprocal lattice can be indexed, are a = 57.16 A, b = 70.85 A, c = 75.81 A, alpha = 85.11 degrees, beta = 121.28 degrees and gamma = 116.33 degrees. There are two Fab fragments comprising the asymmetric unit of the crystals. The presence of a pseudo-mirror plane in the diffraction pattern suggests the presence of at least an approximate dyad axis relating the two Fab fragments within the asymmetric unit.

摘要

一种针对E2特异性猫传染性腹膜炎病毒中和抗体产生的抗体Fab片段，已采用气相扩散法从聚乙二醇4000中结晶出适合X射线衍射分析的形式。该Fab片段晶体的衍射分辨率约为2.9埃，属于三斜晶系空间群P1。可用于标定倒易点阵的晶胞参数为：a = 57.16埃，b = 70.85埃，c = 75.81埃，α = 85.11°，β = 121.28°，γ = 116.33°。晶体的不对称单元包含两个Fab片段。衍射图谱中存在一个假镜面，这表明在不对称单元内至少存在一个近似的二重轴，将两个Fab片段联系起来。