免疫细胞在体外介导牛嗜铬细胞分泌肾上腺素。

Immune cells mediate epinephrine secretion from bovine chromaffin cells in vitro.

作者信息

Jones S B, Wang Z, Wang X, Roberts J C, Weber M, Mathews H L

机构信息

Department of Physiology, Loyola University Medical Center, Maywood, IL 60153.

出版信息

Life Sci. 1993;53(26):PL447-51. doi: 10.1016/0024-3205(93)90022-u.

DOI:10.1016/0024-3205(93)90022-u

PMID:8255153

Abstract

Potential immunological influences on peripheral catecholamine secretion were investigated by measuring epinephrine secretion from chromaffin cells in vitro in response to cell-free conditioned media from mononuclear cells. Chromaffin cells were isolated from bovine adrenals whereas mononuclear cells were isolated from bovine spleen tissue or whole bovine blood. In secretion experiments epinephrine release and epinephrine remaining in cells was determined such that secretion was expressed as % of total cell content. After 90 minutes exposure to conditioned media, 22.8 +/- 1.1% of content was released compared to 1.7 +/- 0.2% with RPMI media. Secretion after filtration (< 3,000 MW cutoff) was 21.6 +/- 0.9% whereas after boiling and boiling in acid, secretion was 10.2 +/- 0.2 and 4.3 +/- 0.1% respectively. Dialysis (< 3,000 MW cutoff) reduced the 90 min conditioned media-stimulated epinephrine secretion from 22.5 +/- 3.8% to 2.3 +/- 0.3%. Neither atropine nor hexamethonium blockade altered the conditioned media-stimulated epinephrine secretion. These results suggest that mononuclear cells produce a low molecular weight substance--most likely a peptide--that contributes to the stimulation of epinephrine secretion.

摘要

通过测量体外嗜铬细胞对来自单核细胞的无细胞条件培养基的反应中肾上腺素的分泌，研究了对外周儿茶酚胺分泌的潜在免疫影响。嗜铬细胞从牛肾上腺中分离出来，而单核细胞从牛脾脏组织或全牛血中分离出来。在分泌实验中，测定肾上腺素的释放量和细胞内剩余的肾上腺素量，以便将分泌量表示为细胞总含量的百分比。在暴露于条件培养基90分钟后，释放的含量为22.8±1.1%，而在RPMI培养基中为1.7±0.2%。过滤（截留分子量<3000）后的分泌量为21.6±0.9%，而煮沸和酸煮后的分泌量分别为10.2±0.2%和4.3±0.1%。透析（截留分子量<3000）将90分钟条件培养基刺激的肾上腺素分泌量从22.5±3.8%降低到2.3±0.3%。阿托品和六甲铵阻断均未改变条件培养基刺激的肾上腺素分泌。这些结果表明，单核细胞产生一种低分子量物质——很可能是一种肽——它有助于刺激肾上腺素分泌。