风疹血凝抑制试验：被细菌污染的血清中的假阳性反应。
Rubella hemagglutination-inhibition test: false-positive reactions in sera contaminated with bacteria.
作者信息
Campbell J B, Romach M, Ellins M L
出版信息
J Clin Microbiol. 1976 Nov;4(5):389-93. doi: 10.1128/jcm.4.5.389-393.1976.
Abstract
We have shown that bacterial contamination of sera can have a marked influence on the results of the rubella hemagglutination-inhibition (HI) test. In addition to increasing the levels of nonspecific agglutinins, a number of common bacterial species tested had a significant effect on the HI titers. When sera free from rubella-specific antibodies were contaminated with Bacillus subtilis or Pseudomonas fluorescens, HI titers ranged from 16 to 128 (expressed as the reciprocal of the highest serum dilution completely inhibiting hemagglutination) after treatment with heparin/MnCl2. Our observations demonstrate, therefore, that bacterial contamination can be one of the causes of false-positive reactions in this test.
摘要
我们已经表明，血清的细菌污染会对风疹血凝抑制（HI）试验的结果产生显著影响。除了增加非特异性凝集素水平外，所检测的一些常见细菌种类对HI滴度有显著影响。当不含风疹特异性抗体的血清被枯草芽孢杆菌或荧光假单胞菌污染时，用肝素/氯化锰处理后，HI滴度范围为16至128（表示为完全抑制血凝的最高血清稀释倍数的倒数）。因此，我们的观察结果表明，细菌污染可能是该试验假阳性反应的原因之一。
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本文引用的文献
1
Modified hemagglutination-inhibition test for rubella employing human group O erythrocytes.
Appl Microbiol. 1972 Mar;23(3):471-5. doi: 10.1128/am.23.3.471-475.1972.
2
Identification of non-specific serum inhibitors of rubella virus haemagglutination.
Med Microbiol Immunol. 1974 Jun 19;159(4):271-7. doi: 10.1007/BF02123736.
3
Use of trypsin-modified human erythrocytes in rubella hemagglutination-inhibition testing.
Appl Microbiol. 1972 Sep;24(3):353-7. doi: 10.1128/am.24.3.353-357.1972.
4
A rubella haemagglutination inhibitor simulating antibody.
Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Dec;81(6):719-23. doi: 10.1111/j.1699-0463.1973.tb02266.x.
5
Rubella hemagglutination inhibition: removal of nonspecific agglutination due to manganous chloride.
Appl Microbiol. 1974 Dec;28(6):992-4. doi: 10.1128/am.28.6.992-994.1974.
6
A rountine diagnostic test for IgA and IgM antibodies to rubella virus: absorption of IgG with Staphylococcus aureus.
J Infect Dis. 1974 Sep;130(3):268-73. doi: 10.1093/infdis/130.3.268.
7
Changes in lipid values and lipoprotein patterns of serum samples contaminated with bacteria.
Am J Clin Pathol. 1973 Jun;59(6):829-35. doi: 10.1093/ajcp/59.6.829.
8
Cell agglutinating activities of some enteric bacteria and effects of various factors on bacteria-tumor cell agglutination.
Sci Rep Res Inst Tohoku Univ Med. 1974 Jul;21(1-2):10-6.
9
Letter: Rubella-specific IgM and a new inhibitor.
Br Med J. 1974 Aug 31;3(5930):579. doi: 10.1136/bmj.3.5930.579-c.
10
Rubella reinfection during pregnancy. A case of mistaken diagnosis of congenital rubella.
Am J Dis Child. 1975 Nov;129(11):1353-6. doi: 10.1001/archpedi.1975.02120480065017.
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