Biano S, Cochran W, Herrmann K L, Hall A D, Chang T W
Am J Dis Child. 1975 Nov;129(11):1353-6. doi: 10.1001/archpedi.1975.02120480065017.
A case of subclinical rubella reinfection during pregnancy with serologic findings in the offspring initially led to an erroneous diagnosis of fetal infection. Laboratory diagnosis of congenital rubella infection, based on finding hemagglutination-inhibition (HI) activity in immunoglobulin M (IgM) fractions of newborn's serum after sucrose gradient fractionation, was questioned when apparent IgM-HI activity was to be probably due to nonspecific inhibitors, which resulted from bacterial action on the beta-lipoprotein in the serum. These findings emphasize some pitfalls encountered in arriving at a diagnosis based soley on serologic data. This case also illustrates the importance of keeping serum samples sterile when performing serologic tests.
一例孕期亚临床风疹再感染病例及其子代的血清学检查结果最初导致了胎儿感染的错误诊断。基于在新生儿血清经蔗糖密度梯度分离后的免疫球蛋白M（IgM）组分中发现血凝抑制（HI）活性来诊断先天性风疹感染，当明显的IgM-HI活性可能是由于非特异性抑制剂所致时受到了质疑，这些非特异性抑制剂是由细菌对血清中β-脂蛋白的作用产生的。这些发现强调了仅基于血清学数据进行诊断时遇到的一些陷阱。该病例还说明了进行血清学检测时保持血清样本无菌的重要性。
