Xu H, Soruri A, Gieseler R K, Peters J H
Department of Immunology, University of Goettingen, Germany.
Scand J Immunol. 1993 Dec;38(6):535-40. doi: 10.1111/j.1365-3083.1993.tb03237.x.
To investigate the differentiation and activation of monocytes, the combined effects of 1,25-dihydroxyvitamin D3 (D3) and IL-4 on human blood monocytes were examined with respect to expression of MHC class-II antigens, accessory activity, and phagocytic capacity. IL-4 was reported to upregulate the expression of MHC class-II antigens and accessory activity of monocytes. The experiments described here demonstrate that D3 inhibits the expression of all three subtypes of MHC class-II antigens (HLA-DR, -DP and -DQ) as well as the accessory activity of monocytes, both in a dose- and time-dependent manner. However, D3 enhances the immunoglobulin- and complement-dependent phagocytosis by monocytes in a dose- and time-dependent manner. When monocytes are treated with both IL-4 and D3, the effects of D3 are reverted by IL-4, suggesting that IL-4 induces the development of monocytes into accessory cells, whereas D3 stimulates differentiation of monocytes into classical macrophages. These findings provide further evidence for the contention that, depending on defined stimuli, monocytes may develop either into accessory cells or into classical macrophages.
为了研究单核细胞的分化和激活，我们检测了1,25-二羟基维生素D3（D3）和白细胞介素-4（IL-4）对人血单核细胞的联合作用，涉及主要组织相容性复合体II类抗原（MHC-II）的表达、辅助活性和吞噬能力。据报道，IL-4可上调MHC-II抗原的表达和单核细胞的辅助活性。本文所述实验表明，D3以剂量和时间依赖性方式抑制MHC-II抗原的所有三种亚型（HLA-DR、-DP和-DQ）的表达以及单核细胞的辅助活性。然而，D3以剂量和时间依赖性方式增强单核细胞对免疫球蛋白和补体依赖性的吞噬作用。当单核细胞同时用IL-4和D3处理时，D3的作用被IL-4逆转，这表明IL-4诱导单核细胞发育为辅助细胞，而D3刺激单核细胞分化为经典巨噬细胞。这些发现为以下观点提供了进一步的证据，即根据特定的刺激，单核细胞可能发育为辅助细胞或经典巨噬细胞。
