Verner K
Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey 17033.
Trends Biochem Sci. 1993 Oct;18(10):366-71. doi: 10.1016/0968-0004(93)90090-a.
Recent in vitro and in vivo experiments suggest that the synthesis and import of mitochondrial proteins are very tightly coupled and that a co-translational import reaction may be mandatory for some proteins. These results are entirely consistent with early experiments which suggested that import occurs co-translationally and that cytosolic polysomes synthesizing mitochondrial proteins are bound to protein import sites on isolated mitochondria. This article discusses and seemingly contradictory reports concerning the involvement of co-translational and post-translational mechanisms in the import process and examines the impact of recent developments in the field.
近期的体外和体内实验表明，线粒体蛋白质的合成与导入紧密耦合，并且共翻译导入反应对于某些蛋白质可能是必需的。这些结果与早期实验完全一致，早期实验表明导入是共翻译发生的，并且合成线粒体蛋白质的胞质多核糖体与分离线粒体上的蛋白质导入位点结合。本文讨论了关于共翻译和翻译后机制在导入过程中所涉及的看似矛盾的报道，并审视了该领域近期进展的影响。
