Fatunmbi O O, Newman J A, Halvorson D A, Sivanandan V
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Avian Dis. 1993 Jul-Sep;37(3):639-46.
The combined effect of time and temperature on the stability of two avian influenza virus (AIV) isolates concentrated with polyethylene glycol (PEG), stored at different temperatures, and used in the preparation of avian influenza vaccine was evaluated in turkeys at 24 hr and at 12, 24, 30, 36, and 42 months of storage. The differences detected between antibodies raised in turkeys by vaccines made from isolates under different storage conditions, times, and temperatures were not significant (P > 0.05), especially with vaccines prepared from one isolate. Virus recovery rates following challenge studies of vaccinated birds were similar. However, birds that were vaccinated twice had lower rates of virus recovery from the trachea, lungs, pancreas, and fecal samples following challenge infection. The results suggest that if stable isolates of AIV can be identified, such isolates can be rapidly concentrated with PEG and stored at -20 C or -196 C for at least 42 months without any loss of potency in the vaccine prepared from these isolates. This would reduce the costs associated with vaccine storage and subsequent expiration dates.
在火鸡中评估了时间和温度对两种用聚乙二醇（PEG）浓缩、保存在不同温度下并用于制备禽流感疫苗的禽流感病毒（AIV）分离株稳定性的联合影响，储存时间为24小时以及12、24、30、36和42个月。由不同储存条件、时间和温度下的分离株制成的疫苗在火鸡中产生的抗体之间检测到的差异不显著（P>0.05），尤其是由一种分离株制备的疫苗。接种疫苗的鸟类攻毒研究后的病毒回收率相似。然而，接种两次疫苗的鸟类在攻毒感染后，从气管、肺、胰腺和粪便样本中回收病毒的比率较低。结果表明，如果能鉴定出稳定的AIV分离株，这些分离株可用PEG快速浓缩，并储存在-20℃或-196℃下至少42个月，而由这些分离株制备的疫苗效力不会有任何损失。这将降低与疫苗储存及后续过期日期相关的成本。
