Devitt C M, Reynolds D L
Department of Microbiology, Immunology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011.
Avian Dis. 1993 Jul-Sep;37(3):749-55.
A rotavirus isolated from the intestinal contents of commercial ring-neck pheasant chicks was determined to be a group D rotavirus based on its RNA electropherotype and its serologic relatedness to known turkey group A and D rotaviruses using immune electron microscopy and agar-gel immunodiffusion assays. Further characterization revealed a buoyant density in cesium chloride of 1.347 g/cm3 for double-shelled particles and 1.365 g/cm3 for single-shelled particles. The average size for the pheasant-origin group D rotavirus was determined to be 80 nm for double-shelled particles and 70 nm for single-shelled particles. In contrast, the average size for a turkey group A rotavirus isolate was determined to be 73 nm for double-shelled particles and 60 nm for single-shelled particles. There was no evidence of viral replication by the pheasant-origin group D rotavirus (either cytopathic effect or increase in numbers of viral particles) after six passages in MA104 cells. The hemagglutinating activity of the pheasant origin group D rotavirus was compared with that of a turkey origin group A rotavirus. Differences and similarities were noted depending upon the species of erythrocytes employed and the temperature of incubation.
从商品环颈雉雏鸡肠道内容物中分离出的一株轮状病毒，根据其RNA电泳图谱以及使用免疫电镜和琼脂凝胶免疫扩散试验检测其与已知火鸡A组和D组轮状病毒的血清学相关性，确定为D组轮状病毒。进一步的特性分析显示，双层颗粒在氯化铯中的浮力密度为1.347 g/cm³，单层颗粒为1.365 g/cm³。源自雉鸡的D组轮状病毒双层颗粒的平均大小确定为80 nm，单层颗粒为70 nm。相比之下，一株火鸡A组轮状病毒分离株双层颗粒的平均大小确定为73 nm，单层颗粒为60 nm。源自雉鸡的D组轮状病毒在MA104细胞中传代6次后，没有病毒复制的证据（无论是细胞病变效应还是病毒颗粒数量增加）。将源自雉鸡的D组轮状病毒的血凝活性与源自火鸡的A组轮状病毒的血凝活性进行了比较。根据所用红细胞的种类和孵育温度，观察到了差异和相似之处。
