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[改良滤纸法用于尿液半定量菌落计数。与计数板的比较(作者译)]

[Improved filter paper method for semiquantitative colony count in urine. Comparison with the counting plate (author's transl)].

作者信息

Pilars de Pilar C E

出版信息

MMW Munch Med Wochenschr. 1976 Nov 5;118(45):1449-52.

PMID:825758
Abstract

A semiquantitative differential colony count of 10(4)-10(9) colonies/ml is quickly prepared by absorbing bacterial suspensions on suitable filter paper strips and subsequent printing on solid media. Single colonies permit firstly the important distinction of mixed and pure cultures, and secondly the immediate resistance test and differentiation. The immersion method using filter paper or agar carriers lacks these two advantages with colonies exceeding 10(5)/ml. Small samples of 1 ml suffice. The reliability and good reproducibility of the filter absorption method were demonstrated by comparison with counting plates, using several hundred samples of pure and mixed cultures of various bacteria with different filter papers and 1024 urine samples with the same type of filter paper. Owing to the small expenditure of material and time, this method is suitable as a screening test in urinary tract infections.

摘要

通过将细菌悬液吸附在合适的滤纸条上,随后印在固体培养基上,可快速制备出每毫升10⁴ - 10⁹个菌落的半定量差异菌落计数。单个菌落首先有助于区分混合培养物和纯培养物,其次可进行即时耐药性测试和鉴别。使用滤纸或琼脂载体的浸泡法在菌落数超过10⁵/ml时缺乏这两个优点。1毫升的小样本就足够了。通过使用不同滤纸对数百份各种细菌的纯培养物和混合培养物样本以及使用同类型滤纸的1024份尿液样本与计数板进行比较,证明了滤膜吸附法的可靠性和良好的重现性。由于材料和时间消耗少,该方法适合作为尿路感染的筛查试验。

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