Heinle H, el Dessouki J
Physiological Institute (I), Tübingen, Germany.
J Biolumin Chemilumin. 1995 Mar-Apr;10(2):71-6. doi: 10.1002/bio.1170100202.
Luminol-enhanced chemiluminescence (LEC) is very sensitive in detecting free radicals but relatively insensitive for hydroperoxides (hydrogen peroxide, tert-butyl hydroperoxide). However, in the presence of opsonized zymosan (often used for stimulation of phagocytic cells) hydroperoxides also induce LEC, suggesting that free radicals are produced under these conditions. Therefore careful interpretation with respect to the nature of the reactive species is necessary when LEC is used for characterization of zymosan-induced phagocytosis. We studied the properties of zymosan-induced LEC under different test conditions and with various inhibitors. Typical radical scavengers, e.g. nordihydroguaiaretic acid and superoxide dismutase, are strong inhibitors, indicating the importance of the superoxide anion. This system is useful for drug testing with respect to antioxidative or radical scavenging activity.
鲁米诺增强化学发光（LEC）在检测自由基方面非常灵敏，但对氢过氧化物（过氧化氢、叔丁基过氧化氢）相对不灵敏。然而，在调理酵母聚糖（常用于刺激吞噬细胞）存在的情况下，氢过氧化物也会诱导LEC，这表明在这些条件下会产生自由基。因此，当使用LEC来表征酵母聚糖诱导的吞噬作用时，有必要对反应性物种的性质进行仔细解读。我们研究了在不同测试条件下以及使用各种抑制剂时酵母聚糖诱导的LEC的特性。典型的自由基清除剂，如去甲二氢愈创木酸和超氧化物歧化酶，是强力抑制剂，这表明超氧阴离子的重要性。该系统对于测试具有抗氧化或自由基清除活性的药物很有用。
