Lojek A, Kozubík A, Cíz M, Hofmanová J
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno.
Gen Physiol Biophys. 1993 Aug;12(4):349-55.
Antioxidative effects of nordihydroguaiaretic acid (NDGA) on murine bone marrow phagocytes were studied using luminol- and lucigenin-amplified chemiluminescence (CL). NDGA applied in vitro strongly suppressed the opsonized zymosan particles stimulated CL response in a dose-dependent manner (in concentrations of 0.3-30 micrograms NDGA/ml), thus confirming its antioxidant activity. However, no effects were observed in mice and their serum samples when investigated one minute to one hour after an i.p. administration of NDGA. These differences suggest that NDGA may undergo a rapid metabolism in vivo.
使用鲁米诺和光泽精增强化学发光法（CL）研究了去甲二氢愈创木酸（NDGA）对小鼠骨髓吞噬细胞的抗氧化作用。体外应用NDGA以剂量依赖性方式强烈抑制调理酵母聚糖颗粒刺激的CL反应（NDGA浓度为0.3 - 30微克/毫升），从而证实了其抗氧化活性。然而，在腹腔注射NDGA后1分钟至1小时进行研究时，在小鼠及其血清样本中未观察到任何影响。这些差异表明NDGA在体内可能会经历快速代谢。
