Effects of nordihydroguaiaretic acid on the chemiluminescence of murine phagocytes.

Antioxidative effects of nordihydroguaiaretic acid (NDGA) on murine bone marrow phagocytes were studied using luminol- and lucigenin-amplified chemiluminescence (CL). NDGA applied in vitro strongly suppressed the opsonized zymosan particles stimulated CL response in a dose-dependent manner (in concentrations of 0.3-30 micrograms NDGA/ml), thus confirming its antioxidant activity. However, no effects were observed in mice and their serum samples when investigated one minute to one hour after an i.p. administration of NDGA. These differences suggest that NDGA may undergo a rapid metabolism in vivo.