Cohen S N, Chang A C, Boyer H W, Helling R B
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4. doi: 10.1073/pnas.70.11.3240.
The construction of new plasmid DNA species by in vitro joining of restriction endonuclease-generated fragments of separate plasmids is described. Newly constructed plasmids that are inserted into Escherichia coli by transformation are shown to be biologically functional replicons that possess genetic properties and nucleotide base sequences from both of the parent DNA molecules. Functional plasmids can be obtained by reassociation of endonuclease-generated fragments of larger replicons, as well as by joining of plasmid DNA molecules of entirely different origins.
本文描述了通过体外连接限制性内切酶产生的不同质粒片段来构建新的质粒DNA种类。通过转化插入大肠杆菌中的新构建质粒被证明是具有生物学功能的复制子，它们拥有来自两个亲本DNA分子的遗传特性和核苷酸碱基序列。功能性质粒既可以通过较大复制子的内切酶产生片段重新缔合获得，也可以通过连接完全不同来源的质粒DNA分子获得。
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