Frenzel H, Kremer B, Richter I E, Hücker H
Res Exp Med (Berl). 1976 Oct 29;168(3):229-41. doi: 10.1007/BF01851321.
The fixation of rat liver by perfusion with glutaraldehyde with different pressures has been investigated. For this study adult male albino rats were used. Rat livers were fixed by perfusion through the abdominal aorta according to the method of FORSSMANN et al. (1967). Perfusion pressures varied from 30 to 210 mmHg. A continuous complete endothelial lining of liver sinusoids could be visualized with TEM and SEM after fixation with perfusion pressures lower than 100 mmHg. Three different regions could be noticed in the endothelial cell: 1. prominent nucleous region, 2. compact cytoplasmic processions containing mitochondria and ergastoplasma, 3. delicate fenestrated cytoplasmic areals. As a rule the fenestrations were localized in groups, s.c. sieve plates. After perfusion fixation with pressures above 100 mmHg the endothelial lining of liver sinusoids appeared similar to a wide-meshed net. The sieve plates were destroyed, and numerous defects could be found in the endothelial cells. Hepatocytes showed vacuoles which seem to be due to invagination of the cellular membrane. For the development of artifacts even with physiological perfusion pressures in the aorta (110 mmHg), the content of procaine in the rinsing solution is responsible. Eliminating the function of arteriols leads to unphysiological pressure effects in the sinusoids.
研究了用不同压力的戊二醛灌注法固定大鼠肝脏的情况。本研究使用成年雄性白化大鼠。按照福斯曼等人（1967年）的方法，通过腹主动脉灌注来固定大鼠肝脏。灌注压力在30至210毫米汞柱之间变化。在用低于100毫米汞柱的压力灌注固定后，用透射电子显微镜（TEM）和扫描电子显微镜（SEM）可以观察到肝血窦连续完整的内皮衬里。在内皮细胞中可以注意到三个不同区域：1. 突出的核区；2. 含有线粒体和内质网的紧密细胞质突起；3. 精细的有窗孔的细胞质区域。通常，窗孔成组定位，即筛板。在用高于100毫米汞柱的压力进行灌注固定后，肝血窦的内皮衬里看起来类似于一个宽网眼的网。筛板被破坏，在内皮细胞中可以发现许多缺陷。肝细胞出现空泡，这似乎是由于细胞膜内陷所致。即使在主动脉中采用生理灌注压力（110毫米汞柱），冲洗液中普鲁卡因的含量也会导致假象的产生。消除小动脉的功能会导致血窦中出现非生理压力效应。
