Ismail T, Ahmad S, D'Souza-Ault M, Bassiri M, Saliki J, Mebus C, Yilma T
Department of Veterinary Microbiology and Immunology, University of California, Davis 95616.
Virology. 1994 Jan;198(1):138-47. doi: 10.1006/viro.1994.1016.
Rinderpest (RP) is a viral disease of ruminants with > 95% morbidity and mortality. We have cloned the cDNA of the nucleocapsid (N) gene of the virulent Kabete O strain of rinderpest virus (RPVK) and compared its nucleotide and deduced amino acid sequences with those of the N genes of the lapinized strain of rinderpest virus (RPVL), measles virus (MV), and canine distemper virus (CDV). The gene has an open reading frame of 1575 nucleotides and encodes a protein of 525 amino acids with a molecular weight of 59 kDa. The nucleotide sequence of the coding region of the N gene of RPVK is 88.6, 68.9, and 63.2% homologous with N genes of RPVL, MV, and CDV, respectively. We have developed a recombinant baculovirus that expresses the N protein (rRVN) of RPVK in insect cells (Sf9) and larvae (Spodoptera frugiperda). rRVN was used as a coating antigen in an ELISA to distinguish vaccinated animals from those infected with RPV and was also used successfully in the diagnosis of two other morbilliviruses, MV and peste des petits ruminants (PPRV). Crude lysate of a single infected larva (0.2-0.3 g) was sufficient to coat 150 ELISA plates for serological diagnosis of 7200 serum samples in duplicate.
牛瘟（RP）是一种反刍动物的病毒性疾病，发病率和死亡率超过95%。我们克隆了牛瘟病毒（RPVK）强毒株卡贝特O株的核衣壳（N）基因的cDNA，并将其核苷酸序列和推导的氨基酸序列与牛瘟病毒兔化株（RPVL）、麻疹病毒（MV）和犬瘟热病毒（CDV）的N基因序列进行了比较。该基因有一个1575个核苷酸的开放阅读框，编码一个525个氨基酸、分子量为59 kDa的蛋白质。RPVK的N基因编码区的核苷酸序列与RPVL、MV和CDV的N基因分别有88.6%、68.9%和63.2%的同源性。我们构建了一种重组杆状病毒，其在昆虫细胞（Sf9）和幼虫（草地贪夜蛾）中表达RPVK的N蛋白（rRVN）。rRVN被用作酶联免疫吸附测定（ELISA）中的包被抗原，以区分接种疫苗的动物和感染RPV的动物，并且还成功用于诊断另外两种麻疹病毒，即MV和小反刍兽疫病毒（PPRV）。单个感染幼虫的粗裂解物（0.2 - 0.3 g）足以包被150块ELISA板，用于对7200份血清样本进行重复血清学诊断。
