Verardi Paulo H, Aziz Fatema H, Ahmad Shabbir, Jones Leslie A, Beyene Berhanu, Ngotho Rosemary N, Wamwayi Henry M, Yesus Mebratu G, Egziabher Berhe G, Yilma Tilahun D
International Laboratory of Molecular Biology for Tropical Disease Agents, Department of Veterinary Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California 95616, USA.
J Virol. 2002 Jan;76(2):484-91. doi: 10.1128/jvi.76.2.484-491.2002.
Rinderpest is an acute and highly contagious viral disease of ruminants, often resulting in greater than 90% mortality. We have constructed a recombinant vaccinia virus vaccine (v2RVFH) that expresses both the fusion (F) and hemagglutinin (H) genes of rinderpest virus (RPV) under strong synthetic vaccinia virus promoters. v2RVFH-infected cells express high levels of the F and H glycoproteins and show extensive syncytium formation. Cattle vaccinated intramuscularly with as little as 10(3) PFU of v2RVFH and challenged 1 month later with a lethal dose of RPV were completely protected from clinical disease; the 50% protective dose was determined to be 10(2) PFU. Animals vaccinated with v2RVFH did not develop pock lesions and did not transmit the recombinant vaccinia virus to contact animals. Intramuscular vaccination of cattle with 10(8) PFU of v2RVFH provided long-term sterilizing immunity against rinderpest. In addition to being highly safe and efficacious, v2RVFH is a heat-stable, inexpensive, and easily administered vaccine that allows the serological differentiation between vaccinated and naturally infected animals. Consequently, mass vaccination of cattle with v2RVFH could eradicate rinderpest.
牛瘟是反刍动物的一种急性且高度传染性的病毒性疾病，通常导致超过90%的死亡率。我们构建了一种重组痘苗病毒疫苗（v2RVFH），该疫苗在强合成痘苗病毒启动子的控制下表达牛瘟病毒（RPV）的融合（F）基因和血凝素（H）基因。v2RVFH感染的细胞表达高水平的F和H糖蛋白，并显示出广泛的合胞体形成。用低至10³ PFU的v2RVFH进行肌肉注射免疫的牛，在1个月后用致死剂量的RPV进行攻毒时，完全受到保护不发生临床疾病；50%保护剂量被确定为10² PFU。用v2RVFH免疫的动物没有出现痘疱病变，也没有将重组痘苗病毒传播给接触的动物。用10⁸ PFU的v2RVFH对牛进行肌肉注射免疫可提供针对牛瘟的长期无菌免疫。除了高度安全和有效外，v2RVFH还是一种热稳定、廉价且易于接种的疫苗，它能够区分接种疫苗的动物和自然感染的动物。因此，用v2RVFH对牛进行大规模免疫接种可以根除牛瘟。
