Ismail T M, Yamanaka M K, Saliki J T, el-Kholy A, Mebus C, Yilma T
Department of Veterinary Pathology, Microbiology and Immunology, University of California, Davis, USA.
Virology. 1995 Apr 20;208(2):776-8. doi: 10.1006/viro.1995.1210.
Peste des petits ruminants (PPR) is a viral disease of goats and sheep characterized by erosive stomatitis, enteritis, and pneumonia. The virus is a member of the family Paramyxoviridae and the genus Morbillivirus. The disease has high morbidity and mortality rates and has a substantial economic impact in developing countries. We have cloned and sequenced the cDNA of the nucleocapsid (N) gene of the Nigeria 75/1 strain of PPR virus (PPRV). A comparison of its nucleotide and deduced amino acid sequence with those of the N gene of the tissue culture-attenuated strain of PPRV was performed. A divergence of 8.9 and 5.0% was found at the nucleotide and amino acid level, respectively. A recombinant baculovirus that expresses the N protein in insect cells and larvae (Spodoptera frugiperda) was generated. The recombinant protein, characterized by Western blot analysis, was shown to have a molecular weight of 58 kDa and was recognized by anti-PPRV antibodies. The recombinant protein was used successfully as a coating antigen in an enzyme-linked immunosorbent assay for the serological diagnosis of PPRV.
小反刍兽疫（PPR）是一种山羊和绵羊的病毒性疾病，其特征为糜烂性口炎、肠炎和肺炎。该病毒属于副粘病毒科麻疹病毒属。这种疾病发病率和死亡率高，在发展中国家造成重大经济影响。我们已克隆并测序了小反刍兽疫病毒（PPRV）尼日利亚75/1株核衣壳（N）基因的cDNA。将其核苷酸和推导的氨基酸序列与PPRV组织培养减毒株的N基因序列进行了比较。在核苷酸和氨基酸水平上分别发现了8.9%和5.0%的差异。构建了一种在昆虫细胞和幼虫（草地贪夜蛾）中表达N蛋白的重组杆状病毒。经蛋白质免疫印迹分析鉴定，该重组蛋白分子量为58 kDa，可被抗PPRV抗体识别。该重组蛋白成功用作酶联免疫吸附试验中的包被抗原，用于PPRV的血清学诊断。
