Khateb A, Fort P, Alonso A, Jones B E, Mühlethaler M
Département de Physiologie, Centre Médical Universitaire, Geneva, Switzerland.
Eur J Neurosci. 1993 May 1;5(5):541-7. doi: 10.1111/j.1460-9568.1993.tb00519.x.
Identified electrophysiologically by low threshold bursts and transient outward rectification, cholinergic nucleus basalis neurons were recorded and labelled intracellularly in guinea-pig basal forebrain slices. By means of a triple labelling immunofluorescent technique, serotonin-immunoreactive fibres were visualized in close proximity to the soma and dendrites of the biocytin-labelled, choline acetyl transferase (ChAT)-immunoreactive cells. By bath application, 5-hydroxytryptamine (5-HT) produced a direct hyperpolarization of the identified cells which was mimicked by 5-HT1A receptor agonists, suggesting that it may inhibit the tonic firing but also modulate the low threshold bursting of the cholinergic nucleus basalis neurons.
通过低阈值爆发和瞬时外向整流在电生理上进行识别，在豚鼠基底前脑切片中对胆碱能基底核神经元进行细胞内记录和标记。通过三重标记免疫荧光技术，在生物胞素标记的、胆碱乙酰转移酶（ChAT）免疫反应性细胞的胞体和树突附近观察到5-羟色胺免疫反应性纤维。通过浴槽给药，5-羟色胺（5-HT）使已识别细胞产生直接超极化，5-HT1A受体激动剂可模拟这种超极化，这表明它可能抑制胆碱能基底核神经元的紧张性放电，还可调节其低阈值爆发。
