Tassopoulos N C, Kuhns M C, Koutelou M G, McNamara A L, Todoulos A
First Department of Medicine, Western Attica General Hospital, Athens, Greece.
Dig Dis Sci. 1993 Dec;38(12):2156-62. doi: 10.1007/BF01299889.
Two hundred forty-four serial serum samples from 30 adults hospitalized with benign (nonfulminant) acute hepatitis B were tested for the presence of hepatitis B virus (HBV) DNA by a quantitative solution hybridization assay using a 125I-labeled DNA probe complementary to HBV-DNA sequences. Acute hepatitis B was self-limiting in 28 and progressed to chronicity in the remaining two patients. Of the 28 patients with self-limiting hepatitis, 21 (75%) were hepatitis B e antigen (HBeAg) positive, 26 (93%) were HBV-DNA positive, and one patient (3.6%) was negative for both markers on admission to the hospital. HBV-DNA cleared after HBeAg clearance in 20 (71.4%), before HBeAg clearance in five (17.9%) and simultaneously with the loss of HBeAg in the remaining two (7.1%) of the 27 initially HBV-DNA- and/or HBeAg-positive patients. Moreover, HBV-DNA remained detectable in serum for 13.3 +/- 6.6 (range: 4-22) days after the appearance of anti-HBe in 71.4% of these patients. In contrast, HBV-DNA and HBeAg remained persistently positive in the two patients who developed chronic HBV infection. These data show that (1) viremia frequently persists after disappearance of HBeAg and (2) appearance of anti-HBe does not indicate the cessation of HBV replication in adults with acute self-limiting hepatitis B.
采用与乙肝病毒（HBV）DNA序列互补的125I标记DNA探针，通过定量溶液杂交试验，对30例因良性（非暴发性）急性乙型肝炎住院的成年人的244份连续血清样本进行了HBV DNA检测。急性乙型肝炎在28例患者中为自限性，其余2例进展为慢性。在28例自限性肝炎患者中，21例（75%）乙肝e抗原（HBeAg）阳性，26例（93%）HBV DNA阳性，1例患者（3.6%）入院时两种标志物均为阴性。在最初HBV DNA和/或HBeAg阳性的27例患者中，20例（71.4%）在HBeAg清除后HBV DNA清除，5例（17.9%）在HBeAg清除前清除，其余2例（7.1%）与HBeAg消失同时清除。此外，71.4%的这些患者在出现抗-HBe后，血清中HBV DNA仍可检测到13.3±6.6（范围：4 - 22）天。相比之下，在发生慢性HBV感染的2例患者中，HBV DNA和HBeAg持续呈阳性。这些数据表明：（1）HBeAg消失后病毒血症常持续存在；（2）在急性自限性乙型肝炎成人患者中，抗-HBe的出现并不表明HBV复制停止。
