一种用于微小隐孢子虫感染的肠道异种移植模型。
An intestinal xenograft model for Cryptosporidium parvum infection.
作者信息
Thulin J D, Kuhlenschmidt M S, Rolsma M D, Current W L, Gelberg H B
机构信息
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana 61801.
出版信息
Infect Immun. 1994 Jan;62(1):329-31. doi: 10.1128/iai.62.1.329-331.1994.
Abstract
Paired segments of near-term fetal rabbit small intestine were transplanted subcutaneously into athymic nude mice. At 5 weeks postsurgery, the xenografts were inoculated intraluminally with Cryptosporidium parvum sporozoites. Parasites rapidly and reliably infected the xenograft mucosal epithelium. Lesions typical of cryptosporidiosis were readily apparent by light microscopy and scanning and transmission electron microscopy. Xenografts are well suited to the study of the early events of C. parvum infection and are of potential value in the evaluation of anticryptosporidial chemotherapeutic agents.
摘要
将近足月胎儿兔小肠的成对节段皮下移植到无胸腺裸鼠体内。术后5周，将微小隐孢子虫子孢子经肠腔接种到异种移植物中。寄生虫迅速且可靠地感染了异种移植物的黏膜上皮。通过光学显微镜、扫描电子显微镜和透射电子显微镜很容易观察到隐孢子虫病的典型病变。异种移植物非常适合研究微小隐孢子虫感染的早期事件，并且在评估抗隐孢子虫化疗药物方面具有潜在价值。
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