Tzipori S, McCartney E, Lawson G H, Rowland A C, Campbell I
Res Vet Sci. 1981 Nov;31(3):358-68.
Piglets from five litters were doses orally with cryptosporidium originally derived from diarrhoeic calves. The piglets were either nursed by the sow, artificially reared after sucking colostrum, or weaned on to creep feed. Colostrum-fed, artificially reared piglets obtained from two litters and exposed in the first week of life developed clinical signs of inappetence, vomiting and diarrhoea and shed oocysts in the faeces. Histologically the parasite was observed throughout the small and large intestine attached to epithelial cell surfaces and its presence was associated with extensive mucosal damage, particularly in the posterior small intestine, stunting and fusion of villi, immaturity of villous epithelial cells and oedema with increased cellularity of the lamina propria. Piglets from two other litters, both sucking and colostrum-fed artificially reared, exhibited similar but milder clinical signs. Histological lesions were less severe and cryptosporidium infection less extensive. When weaned piglets were exposed they remained clinically healthy although histologically there was evidence of cryptosporidium attachment in the small intestine and minor mucosal damage. There appears to be a good correlation between the extent of intestinal infection, the degree of mucosal damage and the severity of clinical disease induced by cryptosporidium in piglets.
选取了五窝仔猪，给它们口服最初源自腹泻犊牛的隐孢子虫。这些仔猪有的由母猪哺乳，有的在吸食初乳后进行人工饲养，还有的断奶后食用仔猪补饲。从两窝中选取的食用初乳、人工饲养的仔猪，在出生第一周接触隐孢子虫后，出现食欲不振、呕吐和腹泻等临床症状，粪便中排出卵囊。组织学检查发现，寄生虫附着于小肠和大肠的上皮细胞表面，其存在与广泛的黏膜损伤有关，尤其是在小肠后部，表现为绒毛发育不良和融合、绒毛上皮细胞不成熟以及固有层细胞增多伴水肿。另外两窝中既吸食母乳又食用初乳、人工饲养的仔猪，表现出类似但较轻的临床症状。组织学损伤较轻，隐孢子虫感染范围较小。断奶仔猪接触隐孢子虫后，虽然组织学检查显示小肠有隐孢子虫附着且有轻微黏膜损伤，但它们在临床上保持健康。仔猪肠道感染的程度、黏膜损伤的程度与隐孢子虫引起的临床疾病严重程度之间似乎存在良好的相关性。
