羟基磷灰石晶体的聚集。

Aggregation of hydroxyapatite crystals.

作者信息

Hansen N M, Felix R, Bisaz S, Fleisch H

出版信息

Biochim Biophys Acta. 1976 Dec 21;451(2):549-59. doi: 10.1016/0304-4165(76)90150-1.

Abstract

A system to study the aggregation of hydroxyapatite crystals was developed. The effect of several factors (Ca2+ x Pi product, Ca2+/Pi ratio, pH, and various substances) were tested. Pb2+, Zn2+, Mg2+ and methyleneblue had only small effects; citrate inhibited aggregation. Pyrophosphate was a strong inhibitor and the diphosphonates disodium ethane-1-hydroxy-1,1-diphosphonate and disodium dichloromethylene diphosphonate were even more potent. The monophosphonate pentanemonophosphonate had no effect. Potent inhibition also occurred with glycosaminoglycans: heparin greater than hyaluronic acid greater than dermatan sulfate greater than chondroitin 4-sulfate greater than chondroitin 6-sulfate. Urine also showed high inhibitory activity. The inhibition of heparin but not that of hyaluronic acid, PPi or urine was abolished by egg white lysozyme. The effects described might be relevant in the normal mineralization process as well as in the mechanisms leading to pathological calcification, such as urinary stone formation.

摘要

开发了一种用于研究羟基磷灰石晶体聚集的系统。测试了几个因素（钙离子与磷酸根离子的乘积、钙磷比、pH值以及各种物质）的影响。铅离子、锌离子、镁离子和亚甲蓝的影响较小；柠檬酸盐抑制聚集。焦磷酸盐是一种强抑制剂，而1-羟基-1,1-二膦酸乙烷二钠和二氯亚甲基二膦酸二钠这两种双膦酸盐的抑制作用更强。单膦酸盐五亚甲基单膦酸盐没有作用。糖胺聚糖也有很强的抑制作用：肝素＞透明质酸＞硫酸皮肤素＞硫酸软骨素4＞硫酸软骨素6。尿液也表现出高抑制活性。蛋清溶菌酶可消除肝素的抑制作用，但不能消除透明质酸、焦磷酸盐或尿液的抑制作用。所描述的这些作用可能与正常矿化过程以及导致病理性钙化（如尿路结石形成）的机制有关。