Houzé P, Chaussard J, Harry P, Pays M
Département de Biochimie-Pharmacologie-Toxicologie, C.H.R. de Versailles, Le Chesnay, France.
J Chromatogr. 1993 Sep 22;619(2):251-7. doi: 10.1016/0378-4347(93)80114-j.
A method has been developed for the separation and measurement of ethylene glycol and three other glycols (propylene glycol, 1,3-butylene glycol and 2,3-butylene glycol) in biological samples by wide-bore column gas chromatography with a flame ionization detector. The method used 1,3-propylene glycol (1,3-propanediol) as an internal standard. The method was linear at least from 2 to 1000 micrograms/ml, with a detection limit of 1 microgram/ml. Analytical recoveries were 89-98% for the different concentrations. Precision studies showed coefficients of variation of 1.5-7.7% for the different concentrations. The assay was applied to the analysis of biological samples from two patients who had ingested ethylene glycol and/or other glycols in a suicide attempt.
已开发出一种方法，通过带有火焰离子化检测器的宽口径柱气相色谱法分离和测量生物样品中的乙二醇以及其他三种二醇（丙二醇、1,3 - 丁二醇和2,3 - 丁二醇）。该方法使用1,3 - 丙二醇作为内标。该方法至少在2至1000微克/毫升范围内呈线性，检测限为1微克/毫升。不同浓度下的分析回收率为89 - 98%。精密度研究表明，不同浓度下的变异系数为1.5 - 7.7%。该测定法应用于分析两名试图自杀而摄入乙二醇和/或其他二醇的患者的生物样品。
