组织提取物中L-蛋氨酸-dl-亚砜的测定

Determination of L-methionine-dl-sulphoxide in tissue extracts.

作者信息

Scislowski P W, Harris I, Pickard K, Brown D S, Buchan V

机构信息

Rowett Research Institute, Bucksburn, Aberdeen, Scotland, UK.

出版信息

J Chromatogr. 1993 Sep 22;619(2):299-305. doi: 10.1016/0378-4347(93)80120-s.

DOI:10.1016/0378-4347(93)80120-s

PMID:8263102

Abstract

The amino acid fraction from rat liver, heart and skeletal muscle was prepared by the separation of sulphosalicylic acid extract on Dowex 50 H+ form. The presence of L-methionine-dl-sulphoxide in these extracts was identified and compared by three independent chromatographic methods: ion-exchange, Pico-Tag and reversed-phase high-performance liquid chromatography after precolumn derivatisation with diethylethoxymethylenemalonate. Quantitative data indicate that L-methionine-dl-sulphoxide is present in the intracellular pool at the levels of free methionine.

摘要

通过在Dowex 50 H+型树脂上分离磺基水杨酸提取物，制备了大鼠肝脏、心脏和骨骼肌的氨基酸组分。采用三种独立的色谱方法（离子交换、Pico-Tag以及用二乙基乙氧基亚甲基丙二酸酯进行柱前衍生后的反相高效液相色谱法）对这些提取物中L-蛋氨酸-dl-亚砜的存在情况进行了鉴定和比较。定量数据表明，L-蛋氨酸-dl-亚砜在细胞内池中的含量与游离蛋氨酸相当。