Windsor B L, Radulovic L L, Bockbrader H N, Chang T
Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, MI 48105.
J Chromatogr. 1993 Sep 22;619(2):315-8. doi: 10.1016/0378-4347(93)80123-l.
A specific and highly sensitive method for the measurement of CI-979 in human plasma is described. The compound and internal standard were extracted from alkalinized plasma with methyl tert.-butyl ether and analyzed by capillary gas chromatography with nitrogen-selective detection. The method was demonstrated to be accurate and precise. Since the limit of quantitation was 0.10 ng/ml, this method was suitable for clinical pharmacokinetic studies in which subjects received repeated administration of 0.5-2.5 mg CI-979 every 6 h.
本文描述了一种测定人体血浆中CI - 979的特异且高灵敏度方法。该化合物及内标物用甲基叔丁基醚从碱化血浆中萃取，并用带氮选择性检测的毛细管气相色谱法进行分析。结果表明该方法准确且精密。由于定量限为0.10 ng/ml，此方法适用于受试者每6小时重复给予0.5 - 2.5 mg CI - 979的临床药代动力学研究。
