采用电子捕获气相色谱法测定血浆中的1,2-苯并异恶唑-3-乙酰氨基肟盐酸盐（PE-257）。

Determination of 1,2-benzisoxazole-3-acetamidoxime hydrochloride (PE-257) in plasma using electron-capture gas chromatography.

作者信息

Yamaguchi T, Utsui Y, Hashimoto M

出版信息

J Chromatogr. 1978 Mar 11;150(1):147-54. doi: 10.1016/s0021-9673(01)92106-5.

Abstract

A gas chromatographic method has been developed that permits the accurate and specific determination of a new psychotropic agent, PF-257, in plasma. PF-257 is extracted with ethyl acetate from alkaline plasma and, after a clean-up procedure, derivatized with heptafluorobutyric anhydride to form 3-[(5-n-heptafluoropropyl-1,2, 4-oxadiazol-3-yl)methyl]-1,2-benzisoxazole (HOMB). The HOMB is assayed on a gas chromatograph equipped with an electron-capture detector. Accurate determinations of PF-257 are possible in the concentration range from 1-40 ng/ml with a relative standard deviation of 6.8%. The minimum detectable concentration in plasma is 0.1 ng/ml. Plasma levels of PF-257 in rats receiving intravenous or oral dosing (10 mg/kg) were determined.

摘要

已开发出一种气相色谱法，可准确、特异性地测定血浆中的一种新型精神药物PF-257。PF-257从碱性血浆中用乙酸乙酯萃取，经过净化程序后，用七氟丁酸酐衍生化，形成3-[(5-正七氟丙基-1,2,4-恶二唑-3-基)甲基]-1,2-苯并异恶唑（HOMB）。HOMB在配备电子捕获检测器的气相色谱仪上进行测定。在1-40 ng/ml的浓度范围内可以准确测定PF-257，相对标准偏差为6.8%。血浆中最低可检测浓度为0.1 ng/ml。测定了接受静脉或口服给药（10 mg/kg）的大鼠血浆中PF-257的水平。