Fulop L, Barrett A D, Phillpotts R, Martin K, Leslie D, Titball R W
Chemical and Biological Defence Establishment, Porton Down, Salisbury, Wiltshire, UK.
J Virol Methods. 1993 Oct;44(2-3):179-88. doi: 10.1016/0166-0934(93)90053-t.
Two conserved regions in the sequence of the NS5 gene of Flaviviruses were identified. Primers were designed from the consensus sequence of these regions and were used in a reverse transcription/polymerase chain reaction (RT/PCR) to amplify a region of the central european tick-borne encephalitis virus Kumlinge NS5 gene. The authenticity of the amplified fragment was confirmed by nucleotide sequencing. A band of the expected size was also obtained when this RT/PCR was applied to 13 other flaviviral RNAs. This method may be useful for characterisation of the NS5 genes of flaviviruses and as a potential pan-flavivirus diagnostic tool.
黄病毒NS5基因序列中的两个保守区域被识别出来。根据这些区域的共有序列设计引物，并将其用于逆转录/聚合酶链反应（RT/PCR），以扩增中欧蜱传脑炎病毒库姆林格株NS5基因的一个区域。通过核苷酸测序证实了扩增片段的真实性。当将此RT/PCR应用于其他13种黄病毒RNA时，也获得了预期大小的条带。该方法可能有助于黄病毒NS5基因的特征分析，并作为一种潜在的泛黄病毒诊断工具。
