Universidade de São Paulo, Faculdade de Medicina, Instituto de Medicina Tropical, Laboratório de Investigação Médica-49, São Paulo, SP, Brasil.
Universidade de São Paulo, Faculdade de Medicina, Instituto de Medicina Tropical, Laboratório de Investigação Médica-46, São Paulo, SP, Brasil.
Rev Soc Bras Med Trop. 2022 Sep 26;55:e0067. doi: 10.1590/0037-8682-0067-2022. eCollection 2022.
Despite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear.
We evaluated the presence of ISFs in mosquitoes collected in São Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction (PCR). Some of the positive samples were subjected to nanopore sequencing.
Twelve mosquito pools (2.8%) tested positive for flavivirus infection. Nanopore sequencing was successfully performed on six samples. Phylogenetic analysis grouped these sequences into genotype 2 of Culex flavivirus (CxFV).
The identification of CxFV genotype 2 at new locations in São Paulo highlights the importance of understanding the role of ISFs in mosquito vector competence.
尽管昆虫特异性黄病毒（ISFs）在世界范围内发生，但它们的分布和作用仍不清楚。
我们使用逆转录和半巢式聚合酶链反应（PCR）评估了在巴西圣保罗采集的蚊子中是否存在 ISFs。一些阳性样本进行了纳米孔测序。
12 个蚊子池（2.8%）检测到黄病毒感染呈阳性。成功对 6 个样本进行了纳米孔测序。系统发育分析将这些序列分为 2 型库蚊黄病毒（CxFV）。
在圣保罗的新地点鉴定出 CxFV 基因型 2，凸显了了解 ISFs 在蚊子媒介能力中的作用的重要性。
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