Leparc I, Fuchs F, Kopecka H, Aymard M
Laboratoire de Virologie, Lyon.
J Clin Microbiol. 1993 Nov;31(11):2890-4. doi: 10.1128/jcm.31.11.2890-2894.1993.
Enteroviruses are common pathogens responsible for a wide spectrum of systemic infections. Conventional diagnosis of these infections relies on the isolation of viruses in cell culture and their identification by seroneutralization with polyclonal or monoclonal antibodies. Among enteroviruses, coxsackieviruses B have been involved as causative agents for viral myocarditis. Most of the time, in the case of cardiac pathologies, viral isolation is negative. Molecular biology techniques appear to be an alternative to conventional diagnosis and could supply evidence for the direct implication of enteroviruses in these severe pathologies. In this paper, we describe a murine experimental model of infection with the presumed highly cardiopathogenic coxsackie-virus B type 3. A kinetics of infection was observed for a period of 31 days, and the classical virological markers (viral isolation from feces and heart biopsies, seroconversion) were monitored and compared by means of molecular techniques (molecular hybridization, polymerase chain reaction [PCR]). In this 31-day period, the detection of coxsackievirus B type 3 RNA in the heart was possible only by using two successive seminested PCRs. After 9 to 11 days of active viral replication, when all other virological markers were negative, positive PCR signals were obtained, which supports the hypothesis of a shift to persistent enteroviral infection.
肠道病毒是引起广泛全身性感染的常见病原体。这些感染的传统诊断依赖于在细胞培养中分离病毒，并通过用多克隆或单克隆抗体进行血清中和来鉴定。在肠道病毒中，柯萨奇病毒B组已被确认为病毒性心肌炎的病原体。大多数情况下，在心脏病变的病例中，病毒分离结果为阴性。分子生物学技术似乎是传统诊断的一种替代方法，可为肠道病毒直接参与这些严重病变提供证据。在本文中，我们描述了一种用假定具有高度心脏致病性的柯萨奇病毒B3型感染的小鼠实验模型。观察了31天的感染动力学，并通过分子技术（分子杂交、聚合酶链反应[PCR]）监测和比较了经典的病毒学标志物（从粪便和心脏活检中分离病毒、血清转化）。在这31天期间，仅通过连续两次半巢式PCR才能在心脏中检测到柯萨奇病毒B3型RNA。在活跃的病毒复制9至11天后，当所有其他病毒学标志物均为阴性时，获得了阳性PCR信号，这支持了向持续性肠道病毒感染转变的假说。
